Journal: The Journal of cell biology

Article Title: DPM1 modulates desmosomal adhesion and epidermal differentiation through SERPINB5.

doi: 10.1083/jcb.202305006

Figure Lengend Snippet: Figure 2. DPM1 modulates DSP localization and cytoskeletal organization. (a) Immunostaining of DSP and DSG2 in HaCaT keratinocytes. Merge indicates overlap between DSP, DSG2, and nuclei stained with DAPI. Scale bar: 10 µm distance. (b) Quantification of the number of DSP puncta over the respective length of cell membrane (µm) from individual cells (represented by individual dots), N = 3. Unpaired Student’s t test. (c) Immunostaining of DSP and DSG2 in primary human keratinocytes. Scale bar: 10 µm distance. Panel shows representatives of three biological replicates. (d) Immunostaining of DSP in 3D-RHE of control (sgNT1) and sgDPM1 conditions. White dashed line indicates insert membrane. Magenta dashed rectangles mark regions magnified on the right (zoomed 2× of original image). Representative of three biological replicates. Scale bar: 10 µm distance. (e–g) Keratin staining depicted by pan-cytokeratin in

Article Snippet: The following primary antibodies were diluted with odyssey blocking buffer in trisbuffered-saline containing 0.1% Tween20 (Thermo Fisher Scientific) and incubated overnight at 4°C, with rotation: mouse Dsc2 mAb (#60239-1-Ig; Proteintech), mouse Dsg2 mAb (clone 10G11, #BM5016; Acris), rabbit Dsg3 pAb (EAP3816; Elabscience), mouse Pkp1 mAb (clone 10B2, #sc-33636; Santa Cruz), mouse Pkp2 mAb (#651101; Progen), mouse PG mAb (clone PG5.1, #61005; Progen), mouse DSP mAb (#sc-390975; Santa Cruz), mouse E-Cad mAb (clone 36, #610181; BD Biosciences), rabbit DSC1 mAb (#ab150382; Abcam), mouse DSG1 (#651111; Progen), rabbit keratin 10 (#905403; BioLegend), mouse keratin 14 (ab7800; Abcam), rabbit DPM1 (#12403-2-AP; Proteintech), rabbit SERPINB5 (MASPIN #ab182785; Abcam), rabbit mGFP (#TA150122; Thermo Fisher Scientific), mouse GAPDH mAb (clone 0411, #sc-47724; Santa Cruz), and mouse α-tubulin (clone 10D8, #627901; BioLegend).

Techniques: Immunostaining, Staining, Membrane, Control

Journal: PLoS ONE

Article Title: Effective Apical Infection of Differentiated Human Bronchial Epithelial Cells and Induction of Proinflammatory Chemokines by the Highly Pneumotropic Human Adenovirus Type 14p1

doi: 10.1371/journal.pone.0131201

Figure Lengend Snippet: Confocal microscopy immunofluorescence analysis of differentiated human bronchial epithelial cells. Figs A, B, C show XY planes, Figs D, E, F show XZ planes. (A, D) Cells were infected from the apical side with HAdV-B14p1 at a moi of 10 (TCID 50 /cell), fixated 4 days p.i. and stained for HAdV in green (FITC conjugated antibody) and the desmoglein 2 (DSG2 receptor) in red (dsRed antibody), the nucleus was counterstained in blue (DAPI). (B, E) Differentiated human bronchial epithelial cells stained for DSG2 receptor in red (dsRed) and occludin (OCLN) a tight junction marker in green (FITC), nucleus was counterstained in blue (DAPI). (C, F) Differentiated human bronchial epithelial cells were stained for CAR receptor in red (dsRed) and the tight junction marker OCLN in green (FITC) and the nucleus in blue (DAPI).

Article Snippet: Primary antibodies used were a polyclonal rabbit occludin (OCLN) antibody (1:40 diluted; Invitrogen, Paisley, UK), a monoclonal mouse CAR antibody (RmcB, 1:100 diluted, kindly provided by M. Bergelson), a monoclonal mouse desmoglein 2 (DSG2) antibody (Clone 6D8, 1:50 diluted, Santa Cruz Biotechnology, Santa Cruz, CA), and a FITC conjugated monoclonal adenovirus antibody (Millipore, Bilerica, MA).

Techniques: Confocal Microscopy, Immunofluorescence, Infection, Staining, Marker

Journal: PLoS ONE

Article Title: Characterization of Desmoglein Expression in the Normal Prostatic Gland. Desmoglein 2 Is an Independent Prognostic Factor for Aggressive Prostate Cancer

doi: 10.1371/journal.pone.0098786

Figure Lengend Snippet: (A) Clear expression of mRNA can be detected for most desmogleins, with the exception of DSG1 which shows only faint expression. ( B–C ) Representative immunofluorescence analyses of DSG2 (B) and DSG4 (C) at the cell border of normal human prostatic glandular epithelium. Original magnification: 200X. Scale bars correspond to 100 µm.

Article Snippet: Anti-DSG2 mouse monoclonal antibody (clone 10G11) was purchased from ARP, Inc (Belmont, MA) and used at a dilution of 1∶50 for immunofluorescence analysis of normal prostate tissue and cell lines; anti-DSG1/2 mouse monoclonal antibody (clone DG3.10) was purchased from Fitzgerald (Acton, MA) and used at a dilution of 1∶20 for TMAs.

Techniques: Expressing, Immunofluorescence

Journal: PLoS ONE

Article Title: Characterization of Desmoglein Expression in the Normal Prostatic Gland. Desmoglein 2 Is an Independent Prognostic Factor for Aggressive Prostate Cancer

doi: 10.1371/journal.pone.0098786

Figure Lengend Snippet: (A–C) DSG2 is expressed in the luminal cells of the prostatic glands and this expression rarely co-localizes with basal cell CK14 expression. ( D–F ) DSG2 co-localizes with PSA expression in the luminal compartment of the gland. ( G–I ) DSG4 is also expressed in the luminal cells and rarely co-localizes with basal cell CK14 expression. ( J–L ) DSG4 expression co-localizes with luminal cell PSA expression. ( M–O ) The expression of DSG4 also shows almost complete co-localization with that of DSG2 in the luminal cells. Original magnification: 200X. Scale bars correspond to 100 µm.

Article Snippet: Anti-DSG2 mouse monoclonal antibody (clone 10G11) was purchased from ARP, Inc (Belmont, MA) and used at a dilution of 1∶50 for immunofluorescence analysis of normal prostate tissue and cell lines; anti-DSG1/2 mouse monoclonal antibody (clone DG3.10) was purchased from Fitzgerald (Acton, MA) and used at a dilution of 1∶20 for TMAs.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Characterization of Desmoglein Expression in the Normal Prostatic Gland. Desmoglein 2 Is an Independent Prognostic Factor for Aggressive Prostate Cancer

doi: 10.1371/journal.pone.0098786

Figure Lengend Snippet: (A) DSG2 expression is detected in all cell lines examined, including the normal immortalized prostate cell line BPH-1. Data is represented as mean ± SD. **P<0.01; *** P<0.001. ( B–E ) DSG2 is expressed at the cell border of LNCaP and DU145 cells; however, cell border expression is not detected in PC3 cells with only some cells showing faint, punctate, and diffuse staining (insert magnification; shown at 2.5X). Original magnification: 200X. Scale bars correspond to 100 µm.

Article Snippet: Anti-DSG2 mouse monoclonal antibody (clone 10G11) was purchased from ARP, Inc (Belmont, MA) and used at a dilution of 1∶50 for immunofluorescence analysis of normal prostate tissue and cell lines; anti-DSG1/2 mouse monoclonal antibody (clone DG3.10) was purchased from Fitzgerald (Acton, MA) and used at a dilution of 1∶20 for TMAs.

Techniques: Expressing, Staining

Journal: PLoS ONE

Article Title: Characterization of Desmoglein Expression in the Normal Prostatic Gland. Desmoglein 2 Is an Independent Prognostic Factor for Aggressive Prostate Cancer

doi: 10.1371/journal.pone.0098786

Figure Lengend Snippet: Expression of DSG2 in prostate cancer as compared to normal prostate.

Article Snippet: Anti-DSG2 mouse monoclonal antibody (clone 10G11) was purchased from ARP, Inc (Belmont, MA) and used at a dilution of 1∶50 for immunofluorescence analysis of normal prostate tissue and cell lines; anti-DSG1/2 mouse monoclonal antibody (clone DG3.10) was purchased from Fitzgerald (Acton, MA) and used at a dilution of 1∶20 for TMAs.

Techniques: Expressing, Standard Deviation

Journal: PLoS ONE

Article Title: Characterization of Desmoglein Expression in the Normal Prostatic Gland. Desmoglein 2 Is an Independent Prognostic Factor for Aggressive Prostate Cancer

doi: 10.1371/journal.pone.0098786

Figure Lengend Snippet: (A–L) Representative immunofluorescence analysis of DSG2 and CK8/18 in prostate cancer. ( D, E–H ) TMAs showing high DSG2 expression in the cell border of well differentiated areas of the tumor (panel D, inside yellow dashed line). ( D, I–L ) TMAs showing low DSG2 expression in poorly differentiated areas of the tumor (panel D, shown in the remainder of the tumor outside of the yellow dashed line). Original magnification: 200X. Scale bars correspond to 100 µm. ( M ) Patients expressing higher levels of DSG2 had a significantly longer recurrence free survival than those expressing lower levels of DSG2 ( P = 0.01).

Article Snippet: Anti-DSG2 mouse monoclonal antibody (clone 10G11) was purchased from ARP, Inc (Belmont, MA) and used at a dilution of 1∶50 for immunofluorescence analysis of normal prostate tissue and cell lines; anti-DSG1/2 mouse monoclonal antibody (clone DG3.10) was purchased from Fitzgerald (Acton, MA) and used at a dilution of 1∶20 for TMAs.

Techniques: Immunofluorescence, Expressing

Journal: PLoS ONE

Article Title: Characterization of Desmoglein Expression in the Normal Prostatic Gland. Desmoglein 2 Is an Independent Prognostic Factor for Aggressive Prostate Cancer

doi: 10.1371/journal.pone.0098786

Figure Lengend Snippet: Correlation between DSG2 and clinico-pathological characteristics.

Article Snippet: Anti-DSG2 mouse monoclonal antibody (clone 10G11) was purchased from ARP, Inc (Belmont, MA) and used at a dilution of 1∶50 for immunofluorescence analysis of normal prostate tissue and cell lines; anti-DSG1/2 mouse monoclonal antibody (clone DG3.10) was purchased from Fitzgerald (Acton, MA) and used at a dilution of 1∶20 for TMAs.

Techniques:

Journal: PLoS ONE

Article Title: Characterization of Desmoglein Expression in the Normal Prostatic Gland. Desmoglein 2 Is an Independent Prognostic Factor for Aggressive Prostate Cancer

doi: 10.1371/journal.pone.0098786

Figure Lengend Snippet: Multivariate analyses in the 414 patient cohort.

Article Snippet: Anti-DSG2 mouse monoclonal antibody (clone 10G11) was purchased from ARP, Inc (Belmont, MA) and used at a dilution of 1∶50 for immunofluorescence analysis of normal prostate tissue and cell lines; anti-DSG1/2 mouse monoclonal antibody (clone DG3.10) was purchased from Fitzgerald (Acton, MA) and used at a dilution of 1∶20 for TMAs.

Techniques: